human duo set elisa kits (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
94
Structured Review
R&D Systems
human duo set elisa kits
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duoset+elisa+kits/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duoset+elisa+kits/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
human duo set elisa kits - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC"
Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
Journal: Redox Biology
doi: 10.1016/j.redox.2026.104172
Figure Legend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control
Figure Legend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot
Related Articles
Enzyme-linked Immunosorbent Assay:Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection Article Snippet: .. The resulting supernatant was used in ELISA assays to measure the production of IL-10, IL-1β, TNF-α, IL-17, CXCL-1, CXCL-2, G-CSF, and GM-CSF using Article Title: Resveratrol therapy modulates inflammatory biomarkers and reverses mechanical allodynia in rats subjected to a temporomandibular joint arthritis model. Article Snippet: Initially, tissue samples were processed with a tissue homogenizer (Ultra Stirrer, Ultra 380) using protease inhibitor cocktail (Roche Diagnostics, Cat. #11836153001) diluted 1:100 in Phosphate Buffered Saline (PBS). .. The spinal cord and brainstem TNF-α, IL-10, and IL-4 levels were evaluated using the Enzyme-Linked Immunosorbent Assay (ELISA) technique with Article Title: Mechanostimulatory Cues Determine Intestinal Fibroblast Fate and Profibrotic Remodeling in a Physiodynamic Human Gut-on-a-Chip. Article Snippet: .. The concentrations of TGF- β, IL-11, and COL1A1 were measured using Article Title: Facilitating siRNA delivery into mammalian cells via the LDL receptor. Article Snippet: .. In addition, serum levels of the cytokines IL-6 and TNF-α were quantified using Article Title: Particle dissolution rate controls macrophage response and drug release from mesoporous silica inhalation carriers. Article Snippet: .. Inflammatory responses of THP-1–derived and primary human macrophages were assessed by measuring the secretion of TNF-α, IL-10, and CCL-2 using Article Title: Early local immune activation following intra-operative radiotherapy in human breast tissue Article Snippet: .. All assays were performed using Article Title: A Rationally Designed Novel Bifunctional Human TNF-α- and Janus Kinase-Targeted soloMER Drug Conjugate (SDC) with a Neutrophil Elastase Cleavable Linker Delivering Inflammation Site-Specific Release of Payload Article Snippet: .. The concentration of mCXCL1 and mMCP-1 was assessed in all serum samples by using Article Title: A Rationally Designed Novel Bifunctional Human TNF-α- and Janus Kinase-Targeted soloMER Drug Conjugate (SDC) with a Neutrophil Elastase Cleavable Linker Delivering Inflammation Site-Specific Release of Payload. Article Snippet: .. The concentration of mCXCL1 and mMCP-1 was assessed in all serum samples by using Bioprocessing:Article Title: Resveratrol therapy modulates inflammatory biomarkers and reverses mechanical allodynia in rats subjected to a temporomandibular joint arthritis model. Article Snippet: Initially, tissue samples were processed with a tissue homogenizer (Ultra Stirrer, Ultra 380) using protease inhibitor cocktail (Roche Diagnostics, Cat. #11836153001) diluted 1:100 in Phosphate Buffered Saline (PBS). .. The spinal cord and brainstem TNF-α, IL-10, and IL-4 levels were evaluated using the Enzyme-Linked Immunosorbent Assay (ELISA) technique with Concentration Assay:Article Title: A Rationally Designed Novel Bifunctional Human TNF-α- and Janus Kinase-Targeted soloMER Drug Conjugate (SDC) with a Neutrophil Elastase Cleavable Linker Delivering Inflammation Site-Specific Release of Payload Article Snippet: .. The concentration of mCXCL1 and mMCP-1 was assessed in all serum samples by using Article Title: A Rationally Designed Novel Bifunctional Human TNF-α- and Janus Kinase-Targeted soloMER Drug Conjugate (SDC) with a Neutrophil Elastase Cleavable Linker Delivering Inflammation Site-Specific Release of Payload. Article Snippet: .. The concentration of mCXCL1 and mMCP-1 was assessed in all serum samples by using |